To decipher the mechanism of Jinzhao Capsules in protecting mice from chronic alcoholic liver injury via the Kelch-like ECH-associated protein-1/nuclear factor erythroid 2-related factor 2/nuclear factor-κB(Keap1/Nrf2/NF-κB) pathway.Methods:Mice were randomized into blank，model，positive drug，and low-，medium-，and high-dose Jinzhao Capsules groups(n=10) according to their body weights.The mice in the other groups except the blank group were administrated with 50% ethanol at a dose of 10 mL/kg by gavage every day.After four consecutive weeks of drug administration，the status of mice was observed，and the activity of transaminase in the serum as well as the levels of ethanol，fat metabolism-related enzymes，and antioxidant enzymes in the liver were measured.In addition，the protein and mRNA levels of factors in the Keap1/Nrf2/NF-κB pathway were determined.The liver tissue was sampled and stained with hematoxylin and eosin，and the pathological changes were examined.Results:Compared with the model group，Jinzhao Capsules groups showed increased transaminase activity in the serum，elevated levels of ethanol，fat metabolism-related enzymes，antioxidant enzymes，and interleukin-10(IL-10) in the liver，up-regulated mRNA level of Nrf2 and protein levels of Nrf2 and heme oxygenase-1(P<0.05，P<0.01)，lowered levels of tumor necrosis factor-α and interleukin-6，and down-regulated mRNA levels of Keap1 and NF-κB p65 and protein levels of Keap1，NF-κB p65，phospho-inhibitory subunit of NF-κB α in the liver(P<0.05，P<0.01).Conclusion:Jinzhao Capsules can protect mice from chronic alcoholic liver injury by enhancing the activities of alcohol metabolism-related enzymes and antioxidant enzymes in the liver tissue and regulating the levels of inflammatory mediators and the mRNA and protein levels of factors in the Keap1/Nrf2/NF-κB pathway.