| 引用本文:贾岚，高晶，李丽，邵紫萱，扈觐玺，刘佳琪，冯广旭，王景霞.金昭胶囊对慢性酒精性肝损伤小鼠的保护作用[J].世界中医药,2024,(12):. |
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| 金昭胶囊对慢性酒精性肝损伤小鼠的保护作用 |
| Jinzhao Capsules Protect Mice from Chronic Alcoholic Liver Injury |
| 投稿时间：2022-07-26 |
| DOI：10.3969/j.issn.1673-7202.2024.12.010 |
| 中文关键词: 金昭胶囊 酒精性肝损伤 乙醇代谢 脂肪代谢 炎症介质 氧化应激 Kelch样环氧氯丙烷相关蛋白1/核因子E2相关因子/核因子κB 小鼠 |
| English Keywords:Jinzhao Capsules Alcoholic liver injury Ethanol metabolism Fat metabolism Inflammatory mediators Oxidative stress Kelch-like ECH-associated protein-1/nuclear factor erythroid 2-related factor 2/nuclear factor-κB(Keap1/Nrf2/NF-κB) Mice |
| 基金项目:国家自然科学基金项目(82074036)——基于调节“环核苷酸-内分泌-细胞因子网络系统”研究白芍养肝阴的物质基础与作用机制；国家中医药管理局中医药行业科研专项(201507004)——含毒性及疑似毒性药材中成药的有效性和安全性文献研究 |
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| 中文摘要: |
| 目的：基于Kelch样环氧氯丙烷相关蛋白1（Keap1）/核因子E2相关因子（Nrf2）/核因子κB通路研究金昭胶囊对慢性酒精性肝损伤小鼠的保护作用。方法：小鼠按体质量随机区组法分为空白组，模型组，阳性药组，金昭胶囊低、中、高剂量组，每组10只。除空白组外，其余各组小鼠每日灌胃50%乙醇10 mL/kg。各组持续给药4周后，观察小鼠状态，测定血清转氨酶活性、肝组织乙醇及脂肪代谢相关酶活性、抗氧化酶活性及Keap1/Nrf2/核因子κB通路相关指标并取肝脏进行苏木精-伊红（HE）病理学检查。结果：与模型组比较，金昭胶囊各剂量组小鼠血清转氨酶活性、肝组织乙醇及脂肪代谢相关酶活性、抗氧化酶水平、炎症介质中白细胞介素-10（IL-10）水平、Nrf2 mRNA水平及Nrf2、血红素加氧酶1（HO-1）表达水平均显著升高(P<0.05、P<0.01)；肝组织炎症介质中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）水平、Keap1mRNA、核因子κB亚基p65亲和肽（NF-κB p65）mRNA水平及Keap1、NF-κB p65和磷酸化核因子κB抑制蛋白α（pIκ-Bα）表达均显著降低(P<0.05、P<0.01)。结论：金昭胶囊具有保护慢性酒精性肝损伤的功效，其作用机制可能与增强肝组织中乙醇代谢酶、抗氧化酶活性，调节炎症介质水平和Keap1/Nrf2/核因子κB通路各指标mRNA及蛋白表达水平相关。 |
| English Summary: |
| To decipher the mechanism of Jinzhao Capsules in protecting mice from chronic alcoholic liver injury via the Kelch-like ECH-associated protein-1/nuclear factor erythroid 2-related factor 2/nuclear factor-κB(Keap1/Nrf2/NF-κB) pathway.Methods:Mice were randomized into blank，model，positive drug，and low-，medium-，and high-dose Jinzhao Capsules groups(n=10) according to their body weights.The mice in the other groups except the blank group were administrated with 50% ethanol at a dose of 10 mL/kg by gavage every day.After four consecutive weeks of drug administration，the status of mice was observed，and the activity of transaminase in the serum as well as the levels of ethanol，fat metabolism-related enzymes，and antioxidant enzymes in the liver were measured.In addition，the protein and mRNA levels of factors in the Keap1/Nrf2/NF-κB pathway were determined.The liver tissue was sampled and stained with hematoxylin and eosin，and the pathological changes were examined.Results:Compared with the model group，Jinzhao Capsules groups showed increased transaminase activity in the serum，elevated levels of ethanol，fat metabolism-related enzymes，antioxidant enzymes，and interleukin-10(IL-10) in the liver，up-regulated mRNA level of Nrf2 and protein levels of Nrf2 and heme oxygenase-1(P<0.05，P<0.01)，lowered levels of tumor necrosis factor-α and interleukin-6，and down-regulated mRNA levels of Keap1 and NF-κB p65 and protein levels of Keap1，NF-κB p65，phospho-inhibitory subunit of NF-κB α in the liver(P<0.05，P<0.01).Conclusion:Jinzhao Capsules can protect mice from chronic alcoholic liver injury by enhancing the activities of alcohol metabolism-related enzymes and antioxidant enzymes in the liver tissue and regulating the levels of inflammatory mediators and the mRNA and protein levels of factors in the Keap1/Nrf2/NF-κB pathway. |
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