| 引用本文:潘福竺1，2，易斌3，4，5，许静2，薛艳华3，5，陈兰3，5，张建永1，张毅2，王单单6，王韵旨6，陈明霞3，4，5，吴宏伟2.炆何首乌乌发的药效评价及其网络药理学机制研究[J].世界中医药,2024,(15):. |
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| 炆何首乌乌发的药效评价及其网络药理学机制研究 |
| Pharmacodynamic Evaluation and Network Pharmacology Mechanism of Stewed Polygoni Multiflori Radix for Hair Blackening |
| 投稿时间：2024-01-21 |
| DOI：10.3969/j.issn.1673-7202.2024.15.002 |
| 中文关键词: 炆制 何首乌 乌发 酪氨酸酶 淋巴增强结合因子1 黑色素生成 Wnt信号通路 氢醌 |
| English Keywords:Stewed Polygoni Multiflori Radix Hair-blackening Tyrosinase Lymphoid enhancer-binding factor 1 Melanin production Wnt signaling pathway Hydroquinone |
| 基金项目:国家自然科学基金青年科学基金项目（82204973）；江西省重点研发计划项目（20223BBG71001）；抚州市“揭榜挂帅”项目（XMBH00031）；中国中医科学院科技创新工程项目(ZZ15-YQ-034，CI2021B015，ZXKT21030) |
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| 中文摘要: |
| 目的：对炆何首乌乌发的药效进行评价，并对其作用机制进行研究。方法：构建小鼠白发模型，通过白发面积在新生毛发区域占比、苏木精-伊红（HE）染色及血浆中酪氨酸酶（TYR）的含量来评价炆何首乌的乌发作用；并利用网络药理学及免疫荧光探讨其作用机制。结果：与假手术组比较，模型组小鼠背部白发面积明显升高，皮肤组织中含黑色素的毛囊数量明显降低，血浆中TYR含量明显降低。与模型组比较，阳性药、制何首乌（中剂量、高剂量）及炆何首乌（低剂量、中剂量、高剂量）均能明显降低小鼠白发面积；制/炆何首乌低剂量、中剂量、高剂量能够明显增加小鼠皮肤组织中黑色素毛囊数量；制/炆何首乌高剂量可以明显提高小鼠血浆中TYR含量。在生药量相同的条件下，与制何首乌比较，炆何首乌中剂量能明显增加黑色素毛囊数量；炆何首乌低剂量、高剂量能明显增加小鼠血浆中TYR含量。网络药理学分析发现，炆何首乌的乌发作用涉及黑色素生成和Wnt信号通路等。免疫荧光结果表明，与假手术组比较，模型组小鼠皮肤组织中淋巴增强结合因子1（LEF1）及TYR表达水平明显降低；炆何首乌组小鼠皮肤组织中LEF1及TYR表达水平均明显增高。结论：炆何首乌对小鼠白发模型具有一定的改善作用，其作用机制可能与上调LEF1及TYR的表达水平有关。 |
| English Summary: |
| To evaluate the hair-blackening efficacy of stewed Polygoni Multiflori Radix(PMR) and investigate its mechanism of action.Methods:A mouse model of white hair was established，and the hair-blackening effect of stewed PMR were assessed by measuring the area of white hair in new hair growth regions，using hematoxylin-eosin(HE) staining，and analyzing plasma tyrosinase(TYR) levels.Network pharmacology and immunofluorescence techniques were employed to explore the mechanism of action.Results:Compared to the sham operation group，the model group exhibited a significant increase in the area of white hair on the back，a notable reduction in melanocyte-containing hair follicles in the skin tissue，and a decrease in plasma TYR levels.Compared with the model group，treatment with positive control，processed PMR(medium and high doses)，and stewed PMR(low，medium，and high doses) significantly reduced the white hair area in mice.Processed and stewed PMR at low，medium，and high doses significantly increased the number of melanocyte-containing hair follicles，while the processed and stewed PMR at a high dose notably increased plasma TYR levels.Under the same dose of crude herb，compared with the processed PMR，the stewed PMR at a medium dose significantly increased the number of melanocyte-containing hair follicles，and stewed PMR at both low and high doses increased plasma TYR levels.Network pharmacology analysis indicated that the hair-blackening effect of stewed PMR involved melanin production and Wnt signaling pathways.Immunofluorescence results showed that，compared with the sham operation group，the model group had significantly reduced expression levels of lymphoid enhancer-binding factor 1(LEF1) and TYR in skin tissue，while the stewed PMR groups exhibited increased levels of LEF1 and TYR expression.Conclusion:Stewed PMR has a notable effect on improving the white hair model in mice，and its mechanism may be related to the upregulation of LEF1 and TYR expression levels. |
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