| 引用本文:李国卫1，胡绮萍1，童培珍1，邱韵静1，曾熙欣2，郭嘉亮2，潘礼业1，何嘉莹1，陈向东1，孙冬梅1.基于氨基酸及特征肽测定的不同产地鸡内金质量研究[J].世界中医药,2024,(15):. |
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| 基于氨基酸及特征肽测定的不同产地鸡内金质量研究 |
| Quality of Galli Gigerii Endothelium Corneum from Different Origins Based on the Determination of Amino Acids and Characteristic Peptides |
| 投稿时间：2024-01-15 |
| DOI：10.3969/j.issn.1673-7202.2024.15.003 |
| 中文关键词: 鸡内金 产地 氨基酸 特征肽 含量测定 偏最小二乘法分析 统计学 质量控制 |
| English Keywords:Galli Gigerii Endothelium Corneum Origin Amino acid Characteristic peptide Content determination Partial least squares discriminant analysis Statistics Quality control |
| 基金项目:国家自然科学基金项目（82373835）——基于全生命周期分析策略的抗细菌生物膜剂高内涵筛选新方法研究；广东省基础与应用基础研究基金项目（2020B1515120033） |
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| 中文摘要: |
| 目的：建立鸡内金氨基酸及特征肽的含量测定方法，对不同产地鸡内金进行质量评价。方法：采用高效液相色谱法对鸡内金的12个氨基酸类成分进行测定，同时采用超高效液相串联三重四级杆质谱法对鸡内金的2个特征肽进行测定，并采用偏最小二乘法分析（PLS-DA）对测定结果进行分析。结果：18批鸡内金的12种氨基酸成分总含量相对标准偏差（RSD）范围为2.84%～14.01%，鸡源多肽Ⅰ、鸡源多肽Ⅱ含量RSD分别为47.13%、14.62%，均呈现一定的波动，PLS-DA分析结果显示，3批产地山西和3批产地辽宁独自分为一类，9批产地山东与3批产地浙江的样品共同分为一类。结论：本研究建立的鸡内金氨基酸及特征肽的含量测定方法，能较好地评价不同产地的鸡内金质量，可为鸡内金及动物类药材的质量评价提供参考。 |
| English Summary: |
| To establish the content determination method of amino acids and characteristic peptides in Galli Gigerii Endothelium Corneum(GGEC) and evaluate the quality of GGEC from different origins.Methods:High-performance liquid chromatography(HPLC) was used to measure the content of 12 amino acids in GGEC，while ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) was employed to determine the levels of two characteristic peptides.Partial least squares discriminant analysis(PLS-DA) was used to analyze the results.Results:The relative standard deviation(RSD) for the total content of the 12 amino acids in 18 batches of GGEC ranged from 2.84% to 14.01%.The RSD for the levels of the two characteristic peptides，Chicken-derived peptide I and Chicken-derived peptide Ⅱ，were 47.13% and 14.62%，respectively，indicating some fluctuations.PLS-DA results showed that three batches of samples from Shanxi and three batches of samples from Liaoning were distinctly grouped into separate categories，while nine batches of samples from Shandong and three batches of samples from Zhejiang were grouped together.Conclusion:The established method for determining amino acids and characteristic peptides in GGEC effectively evaluate the quality of GGEC from different origins and can provide a reference for the quality assessment of GGEC and other animal-based medicinal materials. |
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