| 引用本文:贾岚，刘佳琪，扈觐玺，冯颖童，刘双巧，姜斯佳，冯广旭，王景霞.枳术郁李方对脾-肠气机升降失调便秘小鼠的通便机制研究[J].世界中医药,2024,(15):. |
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| 枳术郁李方对脾-肠气机升降失调便秘小鼠的通便机制研究 |
| Laxative Mechanism of Zhizhu Yuli Formula on Constipation Mice with Spleen-intestine Qi Dysfunction in Ascending and Descending |
| 投稿时间：2023-05-19 |
| DOI：10.3969/j.issn.1673-7202.2024.15.007 |
| 中文关键词: 枳术郁李方 便秘 气机升降 胃肠激素 神经递质 活性肠肽 胃动素 小鼠 |
| English Keywords:Zhizhu Yuli Formula Constipation Qi movement Gastrointestinal hormones Neurotransmitter Vasoactive intestinal peptide Motilin Mice |
| 基金项目:国家重点研发计划中医药现代化研究重点专项（2018YFC1706803）——基于调节“环核苷酸-内分泌-细胞因子网络系统”研究白芍养肝阴的物质基础与作用机制 |
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| 中文摘要: |
| 目的：基于气机升降理论研究枳术郁李方对脾-肠气机升降失调便秘小鼠的通便作用机制，为其临床应用提供实验依据。方法：将雄性昆明小鼠随机分为空白组、模型组、阳性药组、枳术郁李方组，每组10只，阳性药组给予麻仁软胶囊混悬液0.1 g/kg、枳术郁李方组给予枳术郁李方溶液0.483 g/kg，空白组与模型组给予同体积溶剂。连续给药14 d后，采用灌胃给予造模药物盐酸洛哌丁胺（剂量为4 mg/kg），建立小鼠脾-肠气机升降失调便秘模型。测定小肠墨汁推进率，首粒黑便时间，小鼠6 h内黑便粒数及重量，血清血管活性肠肽（VIP）、5-羟色胺（5-HT）、P物质（SP）、一氧化氮（NO）、胃动素（MTL）、胆囊收缩素（CCK）、酪酪肽（PYY）、降钙素基因相关肽（CGRP）的含量。结果：与模型组比较，枳术郁李方组小鼠小肠墨汁推进率明显升高（P<0.01），首便时间明显缩短（P<0.01），6 h内粪便粒数，重量及含水量明显增加(P<0.01)，血清中VIP、NO、CCK、PYY水平明显降低（P<0.01）、SP、CGRP、MTL水平明显升高（P<0.01）。结论：枳术郁李方具有明显的缓解便秘的作用，其作用机制可能与调节脾-肠气机的升降失常，恢复胃肠神经递质和激素的动态平衡，调节气机升降，维持气机畅达相关。 |
| English Summary: |
| To investigate the laxative mechanism of Zhizhu Yuli Formula on constipation mice with spleen-intestine qi dysfunction in ascending and descending based on the theory of qi movement，providing experimental evidence for its clinical application.Methods:Male Kunming mice were randomly divided into a blank group，a model group，a positive drug group，and a Zhizhu Yuli Formula group，with 10 mice in each group.The positive drug group received Maren soft capsule suspension at 0.1 g/kg，the Zhizhu Yuli Formula group received Zhizhu Yuli Formula solution at 0.483 g/kg，and the blank and model groups received an equivalent volume of solvent.After 14 days of continuous administration，the mice were given the modeling drug loperamide hydrochloride(4 mg/kg) via gavage to establish a constipation model with spleen-intestine qi dysfunction in ascending and descending.The small intestine ink propulsion rate，the time to the first black stool，the number and weight of black stools within 6 hours，and the levels of serum vasoactive intestinal peptide(VIP)，5-hydroxytryptamine(5-HT)，substance P(SP)，nitric oxide(NO)，motilin(MTL)，cholecystokinin(CCK)，peptide YY(PYY)，and calcitonin gene-related peptide(CGRP) were measured.Results:Compared with the model group，the Zhizhu Yuli Formula group showed a significant increase in small intestine ink propulsion rate(P<0.01)，a significant reduction in the time to the first stool(P<0.01)，and a significant increase in the number，weight，and water content of fecal pellets within 6 hours(P<0.01).Moreover，the serum levels of VIP，NO，CCK，and PYY were significantly reduced(P<0.01)，while the levels of SP，CGRP，and MTL were significantly increased(P<0.01).Conclusion:Zhizhu Yuli Formula has a significant laxative effect，and its mechanism may be related to regulating the spleen-intestine qi dysfunction in ascending and descending，restoring the dynamic balance of gastrointestinal neurotransmitters and hormones，regulating qi movement，and maintaining smooth qi flow. |
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