| 引用本文:热比姑丽·伊斯拉木1，2，优力都孜·买买提1，2，玉素甫江·艾力1，2，开丽比努尔·阿布来提3，巴合沙拉·马乃甫1，2.土大黄提取物对银屑病小鼠P物质及其神经肽受体-1表达的影响[J].世界中医药,2024,(15):. |
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| 土大黄提取物对银屑病小鼠P物质及其神经肽受体-1表达的影响 |
| Effect of Rumex obtusifolius L.Extract on Substance P and Neurokinin-1 Receptor Expression in Psoriasis Mice |
| 投稿时间：2023-05-06 |
| DOI：10.3969/j.issn.1673-7202.2024.15.009 |
| 中文关键词: 土大黄 银屑病 小鼠 苏木精-伊红染色 实时荧光定量聚合酶链反应 酶联免疫吸附测定 蛋白免疫印迹 P物质 神经肽-1受体 |
| English Keywords:Rumex obtusifolius L Psoriasis Mice HE staining RT-qPCR Elisa Western blot Substance P Neurokinin-1 receptor |
| 基金项目:国家自然科学基金项目（81060314）；新疆维吾尔自治区自然科学基金面上项目（2021D01A158）；新疆维吾尔自治区卫生健康委青年科技人才专项（2015Y40）；新疆维吾尔自治区公益性科研院所专项（KY2015138，KY2015139）；新疆维吾尔自治区重点实验室维吾尔医方剂学实验室开放课题（XJYS1104-2012-04） |
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| 中文摘要: |
| 目的：经土大黄干预，探讨P物质（SP）及其神经激肽-1受体(NK-1R)在银屑病皮肤组织中的表达及意义。方法：收集对照组，银屑病模型组，甲氨蝶呤片组（1.3 mg/kg），阿瑞匹坦胶囊组（5 mg/kg），土大黄组（1、2、4 g/kg）小鼠皮肤、血液标本，经苏木精-伊红（HE）染色，观察银屑病小鼠皮肤组织病理学变化；采用实时荧光定量聚合酶链反应（RT-qPCR）技术，检测银屑病小鼠皮肤SP、NK-1R mRNA表达；采用酶联免疫吸附测定、蛋白免疫印迹技术，检测银屑病小鼠血液SP、皮肤、NK-1R蛋白表达；随后分析SP、NK-1R表达水平与银屑病的关系。结果：与银屑病模型组比较，所有药物干预组均能改善银屑病小鼠皮肤组织病理学变化；同时，小鼠皮肤SP、NK-1RmRNA表达，血清SP、皮肤NK-1R蛋白表达均降低（P<0.05，P<0.01）。与阳性对照药甲氨蝶呤片组、阿瑞匹坦胶囊组比较，1 g/kg大黄组小鼠皮肤组织NK-1R蛋白表达升高（P<0.05）。与土大黄组（1 g/kg）比较，土大黄组（2、4 g/kg）小鼠皮肤组织NK-1R蛋白表达均降低，其中土大黄组（2 g/kg）（P>0.05）、土大黄组（4 g/kg）（P<0.05）。结论：土大黄可能通过降低焦虑相关神经递质SP及其NK-1R受体基因、蛋白表达，从而改善小鼠银屑病样皮损，其作用不亚于阳性对照药甲氨蝶呤片、阿瑞匹坦胶囊。 |
| English Summary: |
| To investigate the expression and significance of substance P(SP) and its neurokinin-1 receptor(NK-1R) in psoriatic skin tissue after intervention with Rumex obtusifolius L.Methods:Skin and blood samples were collected from control mice，psoriasis model mice，and mice treated with methotrexate tablets(1.3 mg/kg)，aprepitant capsules(5 mg/kg)，and R.obtusifolius at doses of 1，2，and 4 g/kg.Hematoxylin and eosin(HE) staining was used to observe the pathological changes in psoriatic skin tissues.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was employed to detect the expression of SP and NK-1R mRNA in psoriatic skin.Enzyme-linked immunosorbent assay(ELISA) and Western blot were used to measure the expression of SP in serum and NK-1R protein in the skin.Subsequently，the relationship between the expression levels of SP，NK-1R，and psoriasis was analyzed.Results:Compared to the psoriasis model group，all drug intervention groups showed improvements in the pathological changes of psoriatic skin tissue and reduced expression of SP and NK-1R mRNA in the skin，and SP in serum，as well as NK-1R protein in the skin(P<0.05，P<0.01).Compared to the positive control drugs methotrexate and aprepitant，the R.obtusifolius group at 1 g/kg showed increased expression of NK-1R protein in the skin tissue(P<0.05).Compared to the results in the R.obtusifolius group at 1 g/kg，the expression of NK-1R protein in the skin tissue was decreased in the R.obtusifolius groups at 2 g/kg and 4 g/kg，with the 2 g/kg group showing a non-significant decrease(P>0.05) and the 4 g/kg group showing a significant decrease(P<0.05).Conclusion:R.obtusifolius may improve psoriasis-like skin lesions in mice by reducing the expression of the anxiety-related neurotransmitter SP and its receptor NK-1R at both the gene and protein levels.Its effectiveness is comparable to the positive control drugs methotrexate and aprepitant. |
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