| 引用本文:牛梦园1，戴卫波1，董更婷1，钟希文1，黄曼婷1，张哲1，王琪文2，李乐愚1.番连化浊方对急性高脂血症小鼠胆固醇代谢紊乱的调节作用[J].世界中医药,2024,(15):. |
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| 番连化浊方对急性高脂血症小鼠胆固醇代谢紊乱的调节作用 |
| Regulatory Effect of Fanlian Huazhuo Formula on Cholesterol Metabolism Disorder in Acute Hyperlipidemia Mice |
| 投稿时间：2023-06-08 |
| DOI：10.3969/j.issn.1673-7202.2024.15.011 |
| 中文关键词: 番连化浊方 高脂血症 胆固醇 去唾液酸糖蛋白受体1 肝X受体α 细胞色素P450 7A1 三磷酸腺苷结合盒转运蛋白A1 三磷酸腺苷结合盒转运蛋白A5 |
| English Keywords:Fanlian Huazhuo Formula Hyperlipemia Cholesterol ASGR1 LXRα CYP7A1 ABCA1 ABCA5 |
| 基金项目:国家自然科学基金青年科学基金项目（82305119）；广东省基础与应用基础研究基金项目（2022A1515011307） |
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| 中文摘要: |
| 目的：探讨番连化浊方对急性高脂血症小鼠胆固醇代谢紊乱的调节作用。方法：采用Triton-WR1339构建C57BL/6小鼠急性高脂血症模型，给药5 d后，测定血清中总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、谷草转氨酶（GOT）、谷丙转氨酶（GPT）含量；苏木精-伊红（HE）染色观察肝脏病理变化；油红O染色检测肝脏内脂质累积情况；蛋白质印迹法检测肝脏去唾液酸糖蛋白受体1（ASGR1）、肝X受体α（LXRα）、三磷酸腺苷结合盒转运蛋白A1（ABCA1）、ABCA5、细胞色素P450 7A1（CYP7A1）蛋白表达变化。结果：与模型组比较，番连化浊方能显著降低血清中TC、TG、LDL-C、GOT、GPT含量，同时升高HDL-C含量（均P<0.05）；显著上调ABCA1、ABCG5、LXRα、CYP7A1蛋白表达水平，显著下调ASGR1蛋白表达水平（均P<0.05）；显著减少肝细胞内脂质累积，改善肝脏病理形态。结论：番连化浊方可能通过调控ASGR1/LXRα/CYP7A1信号通路促进急性高脂血症小鼠的胆固醇外排，降低血脂。 |
| English Summary: |
| To investigate the regulatory effect of Fanlian Huazhuo Formula on cholesterol metabolism disorder in mice with acute hyperlipidemia.Methods:An acute hyperlipidemia model was established in C57BL/6 mice using Triton-WR1339.After 5 days of treatment，serum levels of total cholesterol(TC)，triglycerides(TG)，low-density lipoprotein cholesterol(LDL-C)，high-density lipoprotein cholesterol(HDL-C)，glutamic oxalacetic transaminase(GOT)，and glutamic-pyruvic transaminase(GPT) were measured.Hematoxylin-eosin(HE) staining was used to observe pathological changes in the liver.Oil Red O staining was performed to detect lipid accumulation in the liver.Western blot was used to detect changes in the expression of asialoglycoprotein receptor 1(ASGR1)，liver X receptor α(LXRα)，ATP-binding cassette transporter A1(ABCA1)，ABCA5，and cytochrome P450 7A1(CYP7A1) in the liver.Results:Compared with the model group，Fanlian Huazhuo Formula significantly reduced serum levels of TC，TG，LDL-C，GOT，and GPT，increased HDL-C levels(all P<0.05)，significantly upregulated the protein expression levels of ABCA1，ABCG5，LXRα，and CYP7A1，significantly downregulated the protein expression level of ASGR1(all P<0.05)，significantly reduced lipid accumulation in hepatocytes，and improved liver pathology.Conclusion:Fanlian Huazhuo Formula may promote cholesterol efflux and reduce blood lipids in mice with acute hyperlipidemia by regulating the ASGR1/LXRα/CYP7A1 signaling pathway. |
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