| 引用本文:付佳凤1，王彬1，刁凤胤1，颜嘉怡1，张静1，李艳伟1，张晗1，董天皞2，庄朋伟1，张艳军1，尹清晟1，牛琳3.血必净注射液口服给药改善脓毒症肠屏障的作用机制研究[J].世界中医药,2024,(18):. |
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| 血必净注射液口服给药改善脓毒症肠屏障的作用机制研究 |
| Mechanism of Oral Xuebijing Injection in Improving Intestinal Barrier in Sepsis |
| 投稿时间：2023-08-17 |
| DOI：10.3969/j.issn.1673-7202.2024.18.006 |
| 中文关键词: 血必净 口服给药 脓毒症 肠屏障 给药途径 紧密连接 黏膜通透性 作用机制 |
| English Keywords:Xuebijing Oral administration Sepsis Intestinal barrier Administration route Tight junction Mucosal permeability Mechanism study |
| 基金项目:2023年度国家资助博士后研究人员计划项目（GZC20231925） |
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| 中文摘要: |
| 目的：探讨血必净注射液口服给药对脓毒症小鼠肠屏障功能的保护作用，分析血必净灌胃给药改善肠屏障的潜在作用机制。方法：将雄性ICR小鼠按随机区组法分为对照组（NC）、模型组（M）、血必净灌胃组（XBJ-ig）和血必净静脉注射组（XBJ-iv）；以脂多糖（LPS）阴茎背静脉注射构建脓毒症小鼠肠屏障损伤模型，以生存率、血清中肿瘤坏死因子-α（TNF-α）及白细胞介素1β（IL-1β）的含量、小鼠回肠组织中病理学变化、小鼠肠通透性变化、回肠组织中紧密连接蛋白1（Claudin-1）、小鼠紧密连接蛋白1（ZO-1）的表达进行评价。结果：与模型组比较，血必净灌胃组能够改善脓毒症小鼠死亡率；与对照组比较，模型组小鼠血清中炎症介质TNF-α、IL-1β含量显著升高（P<0.01）；肠通透性显著升高（P<0.01）；Claudin-1、ZO-1蛋白表达量显著下调（P<0.05）。与模型组比较，血必净灌胃组血清中炎症介质TNF-α、IL-1β的水平显著降低（P<0.05）；造模6 h后XBJ-ig组小鼠肠黏膜通透性开始出现显著改善（P<0.05），显著上调Claudin-1、ZO-1蛋白表达（P<0.05）。结论：血必净灌胃给药能够通过改善肠紧密连接蛋白表达，改善脓毒症小鼠的肠屏障功能。 |
| English Summary: |
| To investigate the protective effects of oral administration of Xuebijing Injection on intestinal barrier function in septic mice and analyze the potential mechanisms of oral Xuebijing Injection in improving intestinal barrier function.Methods:Male ICR mice were randomly divided into control group(NC)，model group(M)，oral Xuebijing Injection group(XBJ-ig)，and intravenous Xuebijing Injection group(XBJ-iv) according to ramdom block method.The sepsis-induced intestinal barrier dysfunction model was established by injecting lipopolysaccharide(LPS) into the dorsal penile vein.The study assessed survival rate，serum levels of tumor necrosis factor-alpha(TNF-α) and interleukin-1 beta(IL-1β)，histopathological changes in the ileum，intestinal permeability，and expression of tight junction proteins Claudin-1 and ZO-1 in the ileum.Results:Compared to the model group，the oral Xuebijing Injection group showed an improvement in the survival rate of septic mice.Serum levels of inflammatory mediators TNF-α and IL-1β were significantly increased in the model group compared to the control group(P<0.01)，along with a marked increase in intestinal permeability(P<0.01) and a significant downregulation of Claudin-1 and ZO-1 protein expression(P<0.05).Compared to the model group，the oral Xuebijing Injection group exhibited significantly reduced levels of TNF-α and IL-1β(P<0.05).Six hours after modeling，the intestinal mucosal permeability of the oral Xuebijing Injection group began to show significant improvement(P<0.05)，along with a significant upregulation of Claudin-1 and ZO-1 protein expression(P<0.05).Conclusion:Oral administration of Xuebijing Injection improves intestinal barrier function in septic mice by enhancing the expression of tight junction proteins. |
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