To investigate the effect of Tuoli Xiaodu Powder on the secretion of anti-inflammatory and tissue repair factors in M2 macrophages damaged by high glucose induction.Methods:Human monocytic THP-1 cells were induced to differentiate into M2 macrophages in vitro using phorbol 12-myristate 13-acetate(PMA)，interleukin-4(IL-4)，and interleukin-13(IL-13).The induced cells were identified by observing their morphology and detecting the expression of M2 macrophage surface markers arginase-1(Arg-1) and CD206 via Western blot.Successfully identified M2 macrophages were exposed to 33 mmol/L glucose for 120 h and then randomly divided into an experimental group and a model group.The experimental group was treated with 500 μg/mL Tuoli Xiaodu Powder，while the model group received an equivalent volume of phosphate-buffered saline(PBS).A normal group was also established，receiving the same volume of PBS as an intervention.After 48 h of intervention，the supernatants of the cell cultures were collected，and enzyme-linked immunosorbent assay(ELISA) was used to measure the absorbance of interleukin-10(IL-10)，IL-4，IL-13，transforming growth factor-β1(TGF-β1)，vascular endothelial growth factor(VEGF)，hepatocyte growth factor(HGF)，insulin-like growth factor-1(IGF-1)，platelet-derived growth factor(PDGF)，and fibroblast growth factor(FGF).Results:Microscopy and Western blot analysis confirmed the successful polarization of THP-1 monocytes into M2 macrophages.ELISA results showed that compared to the normal group，the model group exhibited significantly lower levels of IL-10，IL-4，IL-13，VEGF，HGF，IGF-1，TGF-β1，PDGF，and FGF(P<0.05).Compared to the model group，the experimental group showed significantly increased levels of IL-10，IL-4，IL-13，VEGF，HGF，IGF-1，PDGF，and FGF(P<0.05)，while the difference in TGF-β1 levels was not statistically significant.Conclusion:Tuoli Xiaodu Powder promotes the secretion of anti-inflammatory and tissue repair factors in M2 macrophages damaged by high glucose induction，suggesting its potential application in the treatment of diabetic skin ulcer wounds.