To comprehensively evaluate the efficacy and mechanisms of Dachaihu Decoction(DCHD) in different types of cholestasis animal models.Methods:A systematic search was conducted in PubMed，Cochrane Library，Embase，China National Knowledge Infrastructure(CNKI)，China Science Periodical Database(CSPD)，and Chinese Sci-tech Journal Database(CCD) from their inception to April 30，2023，to identify published animal studies on DCHD intervention in cholestasis.Two researchers independently screened reports，extracted relevant data，and assessed the risk of bias using the Systematic Review Center for Laboratory Animal Experimentation(SYRLE) tool and the quality of evidence using the GRADE-CERQual approach.Results:A total of 15 animal studies were included.Except for one study reporting that DCHD failed to alleviate bile duct ligation(BDL)-induced cholestasis，all other studies demonstrated the effectiveness of DCHD in various cholestasis models.The evidence supporting DCHD's efficacy in extrahepatic cholestasis models was stronger than in intrahepatic models.In the analysis of DCHD's mechanisms，the quality of evidence for “inflammation” was rated as high，while “bile acid homeostasis” and “apoptosis” were rated as moderate.Meanwhile，“oxidative stress”“intestinal mucosal barrier”，and “endoplasmic reticulum stress” received low-quality ratings.Conclusion:DCHD has a hepatoprotective effect and alleviates cholestasis in different animal models of cholestasis，with stronger evidence supporting its effects in extrahepatic models.Mechanistically，its anti-inflammatory effect has the highest quality rating，whereas its role in regulating bile acid homeostasis，apoptosis，oxidative stress，intestinal mucosal injury，and endoplasmic reticulum stress has relatively lower evidence quality.