To investigate the antipyretic，anti-inflammatory，analgesic，and microcirculation-improving effects of the Fuzheng Bixie Formula in animal models，and to provide a theoretical basis for its clinical application.Methods:Fever models were established in rats and rabbits using yeast and lipopolysaccharide(LPS) to evaluate the antipyretic effects.The anti-inflammatory effects were assessed using LPS-induced acute lung injury in rats，carrageenan-induced paw edema in rats，and acetic acid-induced increased capillary permeability in mice.Analgesic effects were evaluated using the hot plate test and acetic acid-induced writhing in mice.The effect on microcirculation was evaluated using a carrageenan-induced tail thrombosis model in mice.Results:The Fuzheng Bixie Formula at 2.8 g/kg significantly reduced yeast-induced fever in rats and inhibited LPS-induced fever in rabbits，with statistically significant differences compared to the model group(P<0.05).At doses of 2.8 g/kg and 5.6 g/kg，the formula significantly reduced lung index in LPS-induced lung injury in rats，with statistically significant differences compared to the model group(P<0.05).It also significantly inhibited carrageenan-induced paw edema in rats and acetic acid-induced capillary permeability in mice.Furthermore，the formula demonstrated analgesic effects in both the hot plate and acetic acid-induced writhing tests in mice and significantly reduced tail thrombosis in the carrageenan-induced mouse model，with statistically significant differences compared to the model group(P<0.05).Conclusion:Fuzheng Bixie Formula exhibits significant antipyretic，anti-inflammatory，analgesic，and microcirculation-improving effects.